Journal
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
Volume 133, Issue -, Pages 11-17Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jphotobiol.2014.02.014
Keywords
Hemoglobin; Sodium dodecyl sulfate; Heme degradation; Endogenous hydrogen peroxide; Chemiluminescence; Multivariate curve resolution
Categories
Funding
- University of Tehran, Center of Excellence in Biothermodynamics (CEBiotherm)
- Iran National Elites Foundation (INEF)
- UNESCO Chair in Interdisciplinary Research on Diabetes at University of Tehran
- Iran National Science Foundation (INSF)
Ask authors/readers for more resources
In this study the hemoglobin heme degradation upon interaction with sodium dodecyl sulfate (SDS) was investigated using UV-vis and fluorescence spectroscopy, multivariate curve resolution analysis, and chemiluminescence method. Our results showed that heme degradation occurred during interaction of hemoglobin with SDS producing three fluorescent components. We showed that the hydrogen peroxide, produced during this interaction, caused heme degradation. In addition, the endogenous hydrogen peroxide was more effective in hemoglobin heme degradation compared to exogenously added hydrogen peroxide. The endogenous form of hydrogen peroxide altered oxyHb to aquamethemoglobin and hemichrome at low concentration. In contrast, the exogenous hydrogen peroxide lacked this ability under same conditions. (C) 2014 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available