Journal
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
Volume 125, Issue -, Pages 56-62Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jphotobiol.2013.05.007
Keywords
Copper(II) complex; Tuarine Schiff base; DNA; BSA; Spectroscopy
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Funding
- Natural Science Foundation of Shandong Province of China [Y2004B02]
- Special-purpose Construction Project Foundation for Taishan Scholar of Shandong Province
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The DNA-binding properties and DNA-cleavage activities of a Cu(II) complex, [Cu(sal-tau(phen)]center dot 1.5H(2)O (sal-tau = a Schiff base derived from salicylaldehyde and taurine, phen = 1,10-phenanthroline), have been investigated by using UV-Vis absorption, fluorescence, circular dichroism (CD) spectra and agarose gel electrophoresis. Results indicated that this Cu(II) complex can bind to calf thymus DNA (CT-DNA) via an intercalative mode and shows efficient cleavage activity in the absence and presence of reducer. Its intrinsic binding constant K-b (1.66 x 10(4)M(-1)) was calculated by absorption spectra and its linear Stern-Volmer quenching constant K-sq (3.05) was obtained from florescence spectroscopy, as well as the cleaving reaction rate constant k(1) (2.0 x 10(-4) s(-1)) was acquired from agarose gel electrophoresis. Meanwhile, the interactions of the complex with BSA have also been studied by spectroscopy. Results showed that the complex could quench the intrinsic fluorescence of bovine serum albumin (BSA) remarkably through a static quenching process, and induce a conformational change with the loss of helical stability of protein. (C) 2013 Elsevier B.V. All rights reserved.
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