Journal
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
Volume 120, Issue -, Pages 171-176Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jphotobiol.2012.11.009
Keywords
Breast cancer; Zinc phthalocyanine; Photodynamic therapy
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Funding
- University of Johannesburg, African Laser Centre
- National Research Foundation of South Africa
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The development of curative techniques which are selective for neoplasms is one of the main focal areas in cancer research. The mechanism of cell damage due to Zinc phthalocyanine (ZnPcSmix)-mediated photodynamic therapy (PDT) in a breast cancer cell line (MCF-7) was assessed by inverted light microscopy for morphology, the Trypan blue exclusion assay and adenosine triphosphate (ATP) luminescence assay for cell viability, alamarBlue for proliferation, Lactate Dehydrogenase (LHD) membrane integrity for cytotoxicity and fluorescent microscopy for ZnPcSmix localization. Fluorescent microscopy revealed that ZnPcSmix was localized in both mitochondria and lysosomes, and PDT treated cells showed damaging structural changes and decreased cell viability and proliferation. The light-dependent ZnPcSmix displayed appreciable photosensitivity and the intensity of damage was directly related to its concentration. (C) 2012 Elsevier B.V. All rights reserved.
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