Journal
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
Volume 98, Issue 3, Pages 173-179Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jphotobiol.2009.11.013
Keywords
Singlet oxygen; Photodynamic therapy; Photosensitization; Quencher; Phototoxicity; Time correlated multi photon counting
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Funding
- German Ministry of Education and Science (BMBF)
- Hamamatsu Germany
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The kinetics of chemical singlet oxygen quencher consumption inside living cells during low close illumination was revealed via time resolved singlet oxygen luminescence detection. Deviations of the measured data from the common theoretical model for O-1(2) kinetics forced the authors to consider a one-dimensional diffusion model for description of the kinetics of singlet oxygen generated by membrane localized photosensitizers. Our observations reconcile seemingly contradictory reports presenting different values for the efficiency of singlet oxygen interaction with cells. (C) 2009 Elsevier B.V. All rights reserved.
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