Journal
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
Volume 98, Issue 1, Pages 35-39Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jphotobiol.2009.10.005
Keywords
Fluorophore; Quantum yield; Anisotropy; Fluorescence lifetime
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Funding
- Texas ETF
- AR-PATP [000130-0042-2007]
- Polish Ministry of Science and Higher Education
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We describe spectral properties of novel fluorescence probe DyLight (TM) 594. Absorption and fluorescence spectra of this dye are in the region of Alexa 594 fluor spectra. The quantum yield of DyLight 594 in conjugated form to IgG is higher than corresponding quantum yield of Alexa 594 by about 50%. The new DyLight dye also shows slightly longer lifetime and photostability These favorable properties and high anisotropy value, as well as a high cross-section for two-photon excitation. make this fluorophore attractive as a fluorescence probe in biochemical/biological Studies involving fluorescence methods Published by Elsevier B.V.
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