Antioxidant activity of various solvent extracts from Allomyrina dichotoma (Arthropoda: Insecta) larvae

This study was under taken to evaluate the antioxidant properties of larvae extracts of Allomyrina dichotoma. The antioxidant activities of various larvae extracts of water, methanol, ethyl-acetate, chloroform, and hexane were measured by 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide anion radical and singlet oxygen (O-1(2)). The methanolic larvae extracts displayed the greatest effect in DPPH radical scavenging assay, but the reducing activity of larvae extracts was weaker in the superoxide anion radical assay. However, methanol (ME) and chloroform extracts (CE) could be compared to ascorbic acid in O-1(2) quenching ability. ME (the concentration of 50% O-1(2) quenching, QC(50) = 0.080 mg/ml) and CE (QC(50) = 0.051 mg/ml) extracts had 1.7, 2.7 times better efficiency than ascorbic acid (QC(50) = 0.138 mg/ml), respectively. Also the extracts were found to protect biological systems in Escherichia coli and lactate dehydrogenase against detrimental effects of O-1(2) of type II photosensitization in vitro. The ability of larvae extracts to scavenge free radicals could significantly change contents of GA equivalent, an important factor for the potency of antioxidant capacity. The results suggest that our study may contribute to the development of new bioactive products with potential applications to reduce oxidative stress in living organisms involving reactive oxygen species as well as play a vital role in insect organisms against oxidative damage of undesirable conditions. (C) 2010 Elsevier B.V. All rights reserved.