Journal
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
Volume 219, Issue 2-3, Pages 211-216Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jphotochem.2011.02.012
Keywords
FRET; Phycobiliproteins; Light harvesting complexes; Bilins
Categories
Funding
- FONDECYT [108.0267]
Ask authors/readers for more resources
The high efficiency of energy transfer by phycobiliproteins in phycobilisomes is an interesting and exciting biological phenomenon. The phycobilisome of Gracilaria chilensis contains three phycobiliproteins: phycoerythrin, phycocyanin and allophycocyanin. These proteins have their absorption and emission spectra overlapped allowing a non-radiative, direct and efficient transfer of the excitation energy among them, which is channelled along an energy gradient from the rods to the core and finally transferred to chlorophyll a. Phycobiliproteins are highly fluorescent proteins due to the presence of covalently bound phycobilins which provides them their functional properties. This work reports the experimental determination of the spectroscopic parameters for the chromophores present in R-phycoerythrin of G. chilensis: lifetime, quantum yield, and extinction coefficient of phycourobilin and phycoerythrobilin, using chromophorylated tryptic fragments containing one or two chromophores. These fragments were identified by mass spectrometry and characterized spectroscopically. These measurements provide the individual spectroscopic parameters of the three chromophores (two phycoerythrobilins and one phycourobilin) in the beta subunit and the two chromophores (phycoerythrobilins) bound to the alpha subunit considering the protein environment. (C) 2011 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available