Journal
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
Volume 215, Issue 1, Pages 118-122Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jphotochem.2010.08.008
Keywords
Quantum dots; Bovine serum albumin; Quenching; Fluorescence; Biosensing
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Funding
- CNCSIS [PNII-ID_PCCE_129/2008]
- Agence Universitaire Francophone (AUF)
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In this work we studied the interaction between CdSe/ZnS core-shell quantum dots (QDs) and bovine serum albumin (BSA) protein, and the temperature effects on the structural and spectroscopic properties of both, individual QDs and protein and their bioconjugates (QDs@BSA), by fluorescence and UV-vis spectroscopy. The recorded UV-vis data and the calculated rate of BSA fluorescence quenching by the QDs demonstrated that the interaction between them leads to the formation of QDs@BSA complex. Moreover we show that, compared to the monotonically decrease of the non-conjugated QDs fluorescence intensity, the temperature dependence of the QDs@BSA emission has a much more complex behavior, highly sensitive to the conformational changes of the protein. (C) 2010 Elsevier B.V. All rights reserved.
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