Journal
JOURNAL OF PHARMACY AND PHARMACOLOGY
Volume 66, Issue 6, Pages 844-854Publisher
WILEY
DOI: 10.1111/jphp.12205
Keywords
tomatine; apoptosis; reactive oxygen species; SH-SY5Y cell; neurotoxicity
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Funding
- National Natural Science Foundation of China [31070313]
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Objectives Reactive oxygen species can induce cell apoptosis, and oxidative stress has been implicated in a variety of neurodegenerative disorders. Tomatine, which is a naturally occurring steroidal glycoalkaloid isolated from Solanum cathayanum, has shown potent anti-oxidant properties. Methods In this study, we used the SH-SY5Y cell line as an in vitro model and investigated the protective effect of tomatine against hydrogen peroxide (H2O2)-induced neurotoxicity in SH-SY5Y cells. Key findings Tomatine might inhibit the release of cellular lactate dehydrogenase, increase anti-oxidant enzyme activity and glutathione content, reverse the downregulated protein expression of the brain-derived neurotrophic factor (BDNF), inhibit expression of Bax and activations of caspase-3 and caspase-9 in H2O2-induced SH-SY5Y cells. Conclusions Tomatine exerted beneficially neuroprotective effect on H2O2-induced SH-SY5Y cells, mainly enhancing intracellular anti-oxidant enzyme activity and BDNF expression, inhibiting H2O2-induced oxidative stress as well as expression of Bax and activations of caspase-3 and caspase-9, alleviating H2O2-induced SH-SY5Y cell injury and cell death.
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