Journal
JOURNAL OF PHARMACY AND PHARMACOLOGY
Volume 67, Issue 1, Pages 126-132Publisher
WILEY
DOI: 10.1111/jphp.12317
Keywords
cytoprotection; flow cytometry; human leukemic cells; intracellular K+ concentration; ouabain
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Funding
- Ministry of Education, Culture, Sports, Science and Technology of the Japanese Government
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Objectives We tested if modulation of cytosolic K+ levels by ouabain, an inhibitor of Na+/K+-ATPase, exerts cytoprotection against distinct stressful stimuli in human leukemic cells. Methods The cytosolic K+, Na+ or Ca2+ levels and the cytotoxicity were evaluated by flow cytometry. Key findings Various cytotoxic chemicals and ultraviolet irradiation induced cell death and increased intracellular concentrations of K+, Na+ or Ca2+. Ouabain reduced the cytotoxicity and the elevation of cytosolic levels of K+ but not those of Na+ or Ca2+. Conclusions Our data thus suggest that elevated cytosolic K+ levels are associated with the cytotoxicity in response to distinct stressful stimuli and that ouabain exerts cytoprotection most probably by regulating intracellular K+ levels.
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