Journal
JOURNAL OF PHARMACY AND PHARMACOLOGY
Volume 66, Issue 4, Pages 574-583Publisher
WILEY
DOI: 10.1111/jphp.12139
Keywords
human retinal pigment epithelium cells; monocarboxylate transporter; moxifloxacin
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Funding
- NIH [RO1 EY 09171-16, RO1 EY 10659-14]
- UMKC women's council graduate assistance fund award
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ObjectivesThis work was aim to determine in vitro interaction of moxifloxacin with monocarboxylate transporter (MCT) using a human retinal pigment epithelium cells (ARPE-19). MethodsIn vitro moxifloxacin uptakes were performed at 37 degrees C across ARPE-19 cells. Concentration-dependent uptake of moxifloxacin was performed to delineate moxifloxacin kinetics with MCT. Effects of MCT substrates, MCT inhibitors, pH and metabolic inhibitors on moxifloxacin uptake were conducted to delineate mechanism of moxifloxacin influx via MCT. Key findingsMoxifloxacin uptake was found to exhibit saturable kinetics (Km=1.560.32m and Vmax=0.58 +/- 0.16m/min/mg protein). Higher uptake of moxifloxacin was observed at acidic pH. MCT substrates such as salisylic acid, ofloxacin and L-lactic acid significantly inhibited the uptake of moxifloxacin. Furthermore, moxifloxacin uptake was significantly reduced in the presence of metabolic and MCT inhibitors. Overall, this study demonstrated an interaction of moxifloxacin with Na+ and H+-coupled transporter, most likely MCT1. ConclusionsApart from the lipophilicity, we anticipate that lowest vitreal half-life of intravitreal moxifloxacin compared with other fluoroquinolones may be due to its interaction with MCT. This information might be crucial in clinical settings and can be further explored to improve vitreous half-life and therapeutic efficacy of moxifloxacin.
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