Journal
JOURNAL OF PHARMACY AND PHARMACOLOGY
Volume 64, Issue 4, Pages 597-603Publisher
WILEY-BLACKWELL
DOI: 10.1111/j.2042-7158.2011.01438.x
Keywords
luteolin; migration; proliferation; vascular smooth muscle cells (VSMCs)
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Funding
- Postgraduate Scientific Innovation Research of Jiangsu Province [CX09S_032Z]
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Objectives Luteolin is a naturally occurring flavonoid found in many vegetables, fruits and medicinal plants. The migration and proliferation of vascular smooth muscle cells (VSMCs) are the critical pathological processes in various cardiovascular diseases, such as atherosclerosis. In this study, we investigated the effect of luteolin and its latent mechanism on the proliferation and migration of VSMCs stimulated by hydrogen peroxide (H2O2). Methods VSMC proliferation and cell viability was assayed using the 3-(4,5dimethylthiazol- 2yl)-2,5-diphenyltetrazolium bromide (MTT) method or by cell counting, and H2O2-elicited migration of VSMCs was measured using a transwell migration assay. The phosphorylation levels of Src, 3-phosphoinositide-dependent kinase 1 (PDK1) and Akt (protein kinase B) were analysed by immunoblotting. Key findings This study demonstrated that luteolin showed a particularly inhibitory effect on H2O2-elicited VSMC proliferation and migration. In previous research, we originally explored the function of luteolin in blocking H2O2-triggered Src and Akt signalling pathways. The activation of Src, PDK1, Akt (308), Akt (473) in the luteolin-treated group was significantly lower than that seen in the H2O2 group. Conclusions These findings strongly suggested that luteolin suppresses H(2)O(2)directed migration and proliferation in VSMCs partially due to down-regulation of the Akt and Src signalling pathways, which are important participants in the processes of migration and proliferation of VSMCs.
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