Journal
JOURNAL OF PHARMACY AND PHARMACOLOGY
Volume 62, Issue 12, Pages 1753-1759Publisher
WILEY-BLACKWELL PUBLISHING, INC
DOI: 10.1111/j.2042-7158.2010.01181.x
Keywords
Aloe vera; anti-adhesive; Helicobacter pylori; polysaccharide
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Funding
- National Natural Science Foundation of China [30600117]
- State Key Laboratory of Bio-organic and Natural Products Chemistry of Shanghai Institute of Organic Chemistry of Chinese Academy of Sciences
- State Key Laboratory of Pharmaceutical Biotechnology of Nanjing University [KF-XN-200702]
- Analytical Test Fund of Nanjing University
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Objectives The emergence of antibiotic-resistant Helicobacter pylori strains has necessitated a search for alternative therapies for the treatment of this infection. The aim of this study was to evaluate whether or not polysaccharide fractions from Aloe vera are effective in inhibiting the adherence of H. pylori in vitro. Methods Polysaccharide fractions were extracted from A. vera and subjected to carbohydrate analysis. The adhesive effect was determined by co-incubation of H. pylori and cells with polysaccharides followed by fluorescein isothiocyanate labelling and Gram staining in vitro. Inhibition of H. pylori growth and cellular viability was tested by agar diffusion and MTT assay. Key findings APS-F2 contained significant amounts of galacturonic acid, galactose and arabinose. APS-F1 was galacturonic acid-free and consisted of mannose, glucose and galactose. APS-F2 (0.1, 0.5 and 1.0 mg/ml) reduced the count of H. pylori attached to MKN45 cells to 88, 76 and 64%, respectively. APS-F1 did not show the same effect. Neither polysaccharide revealed an inhibitory effect on the growth of H. pylori or cell viability. In addition, APS-F2 was shown to have a potent anti-adhesive effect against Escherichia coli. Conclusions The results show that the acidic polysaccharide from A. vera has a potent anti-adhesive effect against H. pylori in vitro. However, there have yet to be any in-vivo studies to demonstrate the clinical relevance of this finding.
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