Journal
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
Volume 332, Issue 2, Pages 640-649Publisher
AMER SOC PHARMACOLOGY EXPERIMENTAL THERAPEUTICS
DOI: 10.1124/jpet.109.159871
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Funding
- National Institutes of Health National Center [RR17613]
- Department of Pediatrics, Georgetown University Hospital, Washington, DC
- American Heart Association [AHA00078]
- National Institutes of Health National Heart, Lung, and Blood Institute [R01-HL093429, 073890, HL074940, HL068686, HL092196, R37-HL023081]
- National Institutes of Health National Institute of Diabetes [DK039308]
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Large-conductance, calcium-and voltage-activated potassium (BKCa) channels hyperpolarize coronary artery smooth muscle cells, causing vasorelaxation. Dopamine activates BKCa channels by stimulating D-1-like receptor-mediated increases in cAMP in porcine coronary artery myocytes. There are two D-1-like receptors (R), D1R and D5R. We hypothesize that the specific D-1-like receptor involved in BKCa channel activation in human coronary artery smooth muscle cells (HCASMCs) is the D5R and that activation occurs via cAMP cross-activation of cGMP-dependent protein kinase (PKG), rather than cAMP-dependent protein kinase (PKA). The effects of D-1-like receptor agonists and antagonists on BKCa channel opening in HCASMCs were examined in the presence and absence of PKG/PKA inhibition by cell-attached patch clamp. In the absence of commercially available ligands specific for D1R or D5R, D1R or D5R protein was down-regulated by transfecting HCASMCs with human D1R or D(5)Rantisense oligonucleotides, respectively: cells transfected with scrambled oligonucleotides and nontransfected HCASMCs served as controls. The predominant ion channel conducting outward currents in nontransfected HCASMCs was identified as the large-conductance, calcium-and voltage-activated potassium (BKCa) channel, which was activated by D-1-like receptor agonists despite PKA inhibition with (9R, 10S, 12S)-2,3,9,10,11,12-hexahydro-10- hydroxy-9-methyl-1-oxo-9,12-epoxy-1H-diindolo[1,2,3-fg: 3',2',1'-kl] yrrolo[3,4-i][1,6]benzodiazocine-10-carboxylic acid (KT 5720) (300 nM), but was abolished by inhibiting PKG with 9-methoxy-9- methoxycarbonyl-8-methyl-2,3,9,10-tetrahydro-8,11-epoxy-1H, 8H, 11H-2,7b-11a-triazadibenzo(a,g) cycloocta(cde)-trinden-1-one (KT 5823) (300 nM). D-1-like receptor agonists activated BKCa channels in all transfected cells except those transfected with D 5 R antisense oligonucleotides. Thus, the dopamine (D-1-like) receptor mediates activation of BKCa channels in HCASMCs by D5R, not D1R, and via PKG, not PKA. This is the first report of differential D-1-like receptor regulation of vascular smooth muscle function in human cells.
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