Journal
JOURNAL OF PHARMACOLOGICAL SCIENCES
Volume 119, Issue 3, Pages 193-197Publisher
JAPANESE PHARMACOLOGICAL SOC
DOI: 10.1254/jphs.12R03CP
Keywords
intravital imaging; multiphoton microscopy; cellular dynamics; bone; inflammation
Categories
Funding
- Ministry of Education, Culture, Sports, Science, and Technology of Japan [22689030, 22113007]
- Ministry of Health, Labor and Welfare of Japan [H21-010]
- International Human Frontier Science Program [CDA-00059/2009, RGY0077/2011]
- Grants-in-Aid for Scientific Research [22113007, 22689030] Funding Source: KAKEN
Ask authors/readers for more resources
Intravital multiphoton microscopy has opened a new era in the field of biological imaging. Focal excitation of fluorophores by simultaneous attack of multiple (normally two) photons generates images with high spatial resolution, and use of near-infrared lasers for multiphoton excitation allows penetration of thicker specimens, enabling biologists to visualize living cellular dynamics deep inside tissues and organs without thin sectioning. Moreover, the minimized photo-bleaching and toxicity associated with multiphoton techniques is beneficial for imaging of live specimens for extended observation periods. Here we focus on recent findings using intravital multiphoton imaging of dynamic biological systems such as the immune system and bone homeostasis. The immune system comprises highly dynamic networks, in which many cell types actively travel throughout the body and interact with each other in specific areas. Therefore, real-time intravital imaging represents a powerful tool for understanding the mechanisms underlying this dynamic system.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available