Journal
JOURNAL OF PHARMACOLOGICAL SCIENCES
Volume 108, Issue 3, Pages 290-300Publisher
JAPANESE PHARMACOLOGICAL SOC
DOI: 10.1254/jphs.08101FP
Keywords
calmodulin-dependent protein kinase II (CaMKII); calmodulin; cardiac myocyte; L-type Ca2+ channel
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Funding
- Japan Society for the Promotion of Science
- National Natural Science Foundation of China [30670761]
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The roles of calmodulin (CaM)-dependent protein kinase II (CaMKII) in the maintenance of basal activity and the reversion of run-down of L-type Ca2+ channels were studied in guinea-pig ventricular myocytes by the patch-clamp technique. In the cell-attached configuration, the Ca2+-channel activity was inhibited to 82% - 26% by 1-10 mu M KN-93 and to 92% - 66% by 0.1- 1 mu M autocamtide-2-related inhibitory peptide (AIP) myristoylated. In the inside-out configuration, the bovine cardiac cytoplasm recovered Ca2+-channel activity to 87% of that recorded in the cell-attached configuration, while the CaMKII inhibitor 281-301 at 10 mu M reduced the recovery effect to 19%. CaM + ATP recovered the channel activity to 93% and 28% of that recorded in the cell-attached configuration when applied at 1 and 5 min after run-down, respectively, showing a time-dependent attenuation. However, in the presence of 0.33 mu M CaMKII, this attenuation was abolished, showing 85% and 75% recovery when applied at 1 and 5 min after run-down, respectively. This recovery effect was suppressed by 10 mu M AIP, applied at 5 min, but not at 1 min after run-down. We concluded that CaMKII activation is required in the maintenance of basal activity of L-type Ca2+ channels.
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