Journal
JOURNAL OF PHARMACOLOGICAL SCIENCES
Volume 108, Issue 2, Pages 212-216Publisher
JAPANESE PHARMACOLOGICAL SOC
DOI: 10.1254/jphs.SC0080019
Keywords
ganoderic acid Me (GA-Me); invasion; matrix metalloproteinases 2/9 (MMP2/9)
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Funding
- Shanghai Science & Technology Commission [054319933]
- Shanghai Leading Academic Discipline Project [13203, 13505]
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The effect of ganoderic acid Me (GA-Me), which was purified from the fermentation mycelia of the traditional Chinese medicinal mushroom Ganoderma lucidum as reported (Tang W, Gu TY, Zhong JJ. Biochem Eng J. 2006;32:205-210), on anti-invasion was investigated. Wound healing assay indicated that GA-Me inhibited cell migration of 95-D, a human highly metastatic lung tumor cell line, in dose- and time-dependent manners. Results of cell aggregation and adhesion assays showed that GA-Me promoted cell homotypic aggregation and inhibited cell adherence to extracellular matrix (ECM). In addition, GA-Me suppressed matrix metalloproteinases 2/9 (MMP2/9) gene expressions at both mRNA and protein levels in 95-D cells according to qRT-PCR and Western blotting, respectively. The results demonstrated that GA-Me effectively inhibited tumor invasion, and it might act as a new MMP2/9 inhibitor for anti-metastasis treatment of carcinoma cells.
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