Journal
JOURNAL OF PHARMACOLOGICAL SCIENCES
Volume 108, Issue 1, Pages 124-130Publisher
JAPANESE PHARMACOLOGICAL SOC
DOI: 10.1254/jphs.08128FP
Keywords
resveratrol; rat mesenteric artery; ion channel; vasorelaxation; K+ channel
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Funding
- Ministry of Science and Technology (Serbia)
- Karl & Lora Klein Foundation (Germany)
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Recently it has been suggested that resveratrol relaxes different isolated arteries. The present study addressed the question whether different ion channels are involved in the endothelium-independent mechanism of vasodilatation induced by resveratrol. For that purpose, we tested the action of resveratrol on the rat mesenteric artery without endothelium. Resveratrol induced con centration-dependent relaxation of rat mesenteric artery. Among the K+-channel blockers, 4-amynopiridine (4-AP) moderately antagonized the resveratrol-induced relaxation, while glibendamide, tetraethylammonium chloride, charybdotoxin, margatoxin, and barium chloride did not inhibit resveratrol-induced vasorelaxation. In rings, precontracted with 100 mM K+, the relaxant responses to resveratrol were highly significantly shifted to the right compared to those obtained in rings precontracted with phenylephrine, but resveratrol-induced maximal relaxation was only slightly affected. In order to minimize the influence of K+ channels and voltage-gated Ca2+ channels (VGCCs) in vascular smooth muscle, the third contraction was made by 100 in M K- in the presence of nifedipine. The relaxant response to resveratrol was abolished. Thus, the mechanism of vasorelaxation induced by resveratrol probably involves activation of 4-AP-sensitive K+ channels. Its ability to completely relax the mesenteric artery precontracted with K+-rich solution suggests that K channel-independent mechanism(s) are involved in its vasorelaxant effect. It seems that interaction with VGCCs plays a part in this K+ channel-independent effect of resveratrol.
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