Journal
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Volume 99, Issue -, Pages 83-88Publisher
ELSEVIER
DOI: 10.1016/j.jpba.2014.07.004
Keywords
Gastrodin; p-Hydroxybenzyl alcohol; UFLC-ESI-MS/MS; Pharmacokinetics; metabolite
Categories
Funding
- Program for Changjiang Scholars and Innovative Research Team in University [PCSIRT-IRT1193]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
Ask authors/readers for more resources
A simple, sensitive and reliable ultra fast liquid chromatography-electrospray ionization-tandem mass spectrometry (UFLC-ESI-MS/MS) method was developed for simultaneously quantifying gastrodin (p-hydroxy-methyl-phenol-beta-D-glucoside) and its metabolite p-hydroxybenzyl alcohol (HBA) in dog plasma. Separation was performed on an ultra fast liquid chromatography (UFLC) system. Detection was carried out on a tandem mass spectrometry (MS/MS) in multiple reaction monitoring (MRM) mode via an electrospray ionization (ESI) interface. MRM mode of precursor-product ion transitions was used for gastrodin, HBA and the internal standard (IS, bergeninum) at m/z 285.0 -> 123.0, 123.0 -> 105.0 and 326.9 -> 192.2, respectively. The lower limits of quantification (LLOQ) of this method for both gastrodin and HBA were 1 ng/mL, with their linear concentration ranging from 0.001 to 10 mu g/mL. The methods were validated for selectivity, calibration curves, accuracy and precision, extraction recoveries, matrix effects, carry-over, cross talk, dilution integrity, stability and incurred sample reanalysis (ISR). Using this validated method, pharmacokinetic behaviors of gastrodin and HBA after intragastric administration (ig) of gastrodin to dogs were studied for the first time. (C) 2014 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available