Journal
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Volume 100, Issue -, Pages 271-278Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jpba.2014.08.008
Keywords
Dispersive liquid-liquid microextraction; High-performance liquid chromatography-ultraviolet detection; Response surface methodology; Tricyclic antidepressants; Urine analysis
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Funding
- Razi University, Kermanshah, Iran
- Islamic Azad University, Shahreza Branch, Iran
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Dispersive liquid-liquid microextraction (DLLME) coupled with high performance liquid chromatography by ultraviolet detection (HPLC-UV) as a fast and inexpensive technique was applied to the determination of imipramine and trimipramine in urine samples. Response surface methodology (RSM) was used for multivariate optimization of the effects of seven different parameters influencing the extraction efficiency of the proposed method. Under optimized experimental conditions, the enrichment factors and extraction recoveries were between 161.7-186.7 and 97-112%, respectively. The linear range and limit of detection for both analytes found to be 5-100 ng mL(-1) and 0.6 ng mL(-1), respectively. The relative standard deviations for 5 ng mL-1 of the drugs in urine samples were in the range of 5.1-6.1 (n = 5). The developed method was successfully applied to real urine sample analyses. (C) 2014 Elsevier B.V. All rights reserved.
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