Journal
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Volume 75, Issue -, Pages 25-32Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jpba.2012.11.009
Keywords
Bioassay-guided fractionation; HPLC-ESI-Q/TOF-MS; HSCCC; Puerariae thomsonii Flos; Tectorigenin
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Funding
- Research and Innovation Project for College Graduates of Jiangsu Province [CXZZ11-0810]
- Program for the Top Doctor Innovative Talents of China Pharmacuetical University [2011IIBPY03]
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In this study, an efficient strategy based on bioassay-guided fractionation, high-performance liquid chromatography/electrospray ionization quadrupole time-of-flight mass spectrometry (HPLC-ESI-Q/TOF-MS) and high-speed counter-current chromatography (HSCCC) was established to screen and purify bioactive compounds from Chinese herbal medicines (CHMs). This screening system was efficient and successfully applied to reveal anti-prostate cancer candidates from Puerariae thomsonii Flos. As a result, an active fraction with strong in vitro anti-prostate cancer activity was obtained, and the main compounds in the fraction were purified by HSCCC, giving 82 mg of tectoridin, 36 mg of tectorigenin-7-O-[beta-D-xylopyranosyl-(1 -> 6)-beta-D-glucopyranoside and 64 mg of tectorigenin. Among them, tectorigenin, possessing the highest anti-prostate cancer activity with IC50 value of 0.08 mu M, has priority to be lead compound. The results of this work demonstrated that the developed method was efficient and could be employed for the rapid screening, identification and purification of active components from CHMs. (C) 2012 Elsevier B.V. All rights reserved.
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