Journal
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Volume 77, Issue -, Pages 71-75Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jpba.2012.12.031
Keywords
Radix angelicae pubescentis; Coumarins; Columbianetin acetate; Osthole; LC-MS/MS
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Funding
- Doctoral Fund of Ministry of Education of China [20101210120007]
- National Natural Science Foundation of China [81001632]
- China Post-doctoral Science Foundation [20100480657]
- Key Project of Chinese Ministry of Education [210013]
- Tianjin Research Program of Application Foundation and Advanced Technology [12JCQNJC08800]
- Program for Changjiang Scholars and Innovative Research Team of the University (PCSIRT)
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A rapid and sensitive bioassay based on liquid chromatography tandem mass spectrometry (LC-MS/MS) has been developed and validated for the simultaneous determination of eight coumarins in rat plasma. The liquid-liquid extraction method with ethyl acetate was used to prepare the plasma samples after addition of warfarin as an internal standard (IS). Chromatographic separation was performed on an Eclipse plus C18 column (100 mm x 4.6 mm, 1.8 mu m) using gradient elution when 1 mM ammonium acetate aqueous solution - acetonitrile was used as the mobile phase. The lower limit of quantitation (LLOQ) of each coumarin was lower than 2.16 ng mL(-1). Intra-day and inter-day precisions were less than 15%. The accuracies were in the range of 88.9-117%. The mean recoveries of coumarins and IS were higher than 84%. The method was successfully applied to a pharmacokinetic study of eight coumarins in rats after oral administration of radix angelicae pubescentis. Crown Copyright (c) 2013 Published by Elsevier B.V. All rights reserved.
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