Journal
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Volume 77, Issue -, Pages 83-87Publisher
ELSEVIER
DOI: 10.1016/j.jpba.2013.01.015
Keywords
Absolute ionic mobility; Acidity constant; Amino acid; Cationic coating; Hydrodynamic radius
Categories
Funding
- Natural Science Foundation of Gansu Province, China [0803RJZ017]
- National Science Foundation of China [21175143, 201105107]
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The ionization constant (pK(a)) and limiting ionic mobility of 3-chlorotyrosine (CT) and 3-nitrotyrosine (NT) were determined in capillary zone electrophoresis (CZE) in a wide pH range. Measurements were carried out in a poly(ionic liquid) (PIL) modified capillary at a low pH (1.80-4.00) and a bare fused capillary at an upper pH (3.00-11.00). Electrostatic interaction between analytes and inner wall was suppressed dramatically. Furthermore, parameters usually empirically assumed were calculated from a simple theoretical model. Besides pK(a) and limiting ionic mobility, diameter of the hydrated ion was calculated as well. The former were inserted into the database of Peakmaster software, whose predictions led to satisfactory agreement with experimental runs. (c) 2013 Elsevier B.V. All rights reserved.
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