Journal
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Volume 54, Issue 1, Pages 186-191Publisher
ELSEVIER
DOI: 10.1016/j.jpba.2010.07.010
Keywords
Flavonoid; Herba Epimedii; Er-Xian Decoction; Dog plasma; Ultra-performance liquid chromatography-tandem mass spectrometry; Bioequivalence study
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Funding
- Ministry of Science and Technology of the People's Republic of China [2009ZX09301-003-5-1]
- Ministry of Education of the People's Republic of China
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In this study, a sensitive and specific ultra-performance liquid chromatography-tandem mass method has been developed and validated for the identification and determination of 7 flavonoids in dog plasma for the first time: epimedin A, epimedin B, epimedin C, icariin, sagittatoside B, 2 ''-O-rhamnosyl icariside II, and baohuoside I. Chromatographic separation was accomplished on an Agilent Zorbax-SB C-18 column (50 mm x 2.1 mm, 1.8 mu m) with a gradient elution system composed of 0.3% acetic acid and 0.3% acetic acid in acetonitrile at a flow rate of 0.4 mL/min. Detection was based on a triple quadrupole mass spectrometer using a multiple reaction monitoring mode with an electrospray ionization source. All of the calibration curves showed good linearity (r> 0.99) within the tested concentration ranges. The lower limits of quantification of the seven analytes were all lower than 0.0654 ng/mL.. The relative standard deviations for intra- and inter-batches of the seven analytes were less than 13.7% and 14.9%, respectively, at four concentration levels of quality control samples, and the recoveries were between 92.8% and 114.5%, respectively. In addition, the seven flavonoids were found to be stable in dog plasma samples under short- and long-term storage and processing conditions. The validated method was successfully applied to a bioequivalence study in dog plasma after the oral administration of extracts of Herba Epimedii and Er-Xian Decoction. (c) 2010 Elsevier B.V. All rights reserved.
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