Journal
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Volume 53, Issue 3, Pages 785-789Publisher
ELSEVIER
DOI: 10.1016/j.jpba.2010.05.018
Keywords
High-performance liquid chromatography; Monolithic column; Parabens; Saliva; Toothpaste; Solid phase extraction
Categories
Ask authors/readers for more resources
The present study reports the development and application of an HPLC-UV method for the simultaneous separation and determination of five paraben preservatives (methyl-, ethyl-, propyl-, n-butyl- and iso-butyl-paraben) in real samples All analytes were separated efficiently in less than 20 min using a simple H2O center dot ACN linear gradient and a short monolithic column (50 mm x 4.6 min i.d) at a flow rate of 3 0 mL min(-1). Phenoxyethanol was used as chromatographic internal standard The method was validated for linearity, limits of detection and quantification, accuracy and precision Human saliva and toothpaste samples were analyzed after SPE pretreatment on Licrolut RP-18 cartridges The detection limits varied between 0.1 and 0 3 mg L-1 in all cases and the percent recoveries between 86 and 113% (C) 2010 Elsevier B V All rights reserved
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available