Journal
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Volume 53, Issue 4, Pages 1042-1047Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jpba.2010.06.030
Keywords
Sirolimus; Dog blood; Basic mobile phase; Pharmacokinetics; LC-MS/MS
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Funding
- Ministry for Health, Welfare and Family Affairs, Republic of Korea [A080470]
- Korea Health Promotion Institute [A080470] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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A rapid, sensitive method of detecting sirolimus in blood was developed and applied in pharmacokinetic studies employing deionized water for hemolysis and a weakly basic mobile phase to enhance chromatographic peak intensity. Dog blood samples were processed via liquid-liquid extraction and the amounts of sirolimus and tacrolimus, an internal standard, were quantified by LC-MS/MS. Specificity, the lower limit of quantification, linearity, accuracy, precision, dilution, recovery, matrix effects, robustness and stability were within the acceptable range for assay validation. The concentration of sirolimus was quantifiable in blood samples for up to 36 h after the dog had received a 3 mg/kg dose of sirolimus. These observations suggest that sirolimus can be detected at low levels in dog blood using a basic mobile phase and metal-free hemolysis. This method is therefore applicable to pharmacokinetic studies in dogs. (C) 2010 Elsevier B.V. All rights reserved.
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