Journal
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Volume 51, Issue 5, Pages 1169-1174Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jpba.2009.11.030
Keywords
Paclitaxel; Poly(L-glutamic acid)-alanine-paclitaxel conjugate; Rat plasma; Liquid chromatography-tandem mass spectrometry Pharmacokinetics
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Funding
- National Key Technologies R & D Program for New Drugs [2009ZX09301-002, 2009ZX09304-004, 2009ZX09103-130]
- National Natural Science Foundation of China [30672507]
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A specific and sensitive liquid chromatography-tandem mass spectrometric method for quantitative determination of paclitaxel in rat plasma was developed and validated using docetaxel as an internal standard. Liquid-liquid extraction using tert-butyl methyl ether was used to extract the drug and the internal standard from plasma. The separation of paclitaxel was performed on a C(18) column with a mobile phase of acetonitrile:water: formic acid (65:35:0.1, v/v/v) over 5 min. The assay was based on the selected reaction monitoring transitions at m/z of the precursor-product ion transitions m/z 854.2 -> 286.1 for paclitaxel and 808.3 -> 527.2 for internal standard. The lower limit of quantification was 0.5 ng/mL based on 100 mu L of plasma. Intra- and inter-day assay variations were less than 15%, and the accuracy values were between 95.4 and 105.4%. The extraction recoveries ranged from 96.7 to 103.7% across the calibration curve range. The method was successfully applied to measurement of low concentrations of paclitaxel or regenerated paclitaxel in plasma after intravenous administration of a single dose (10 mg/kg) of a poly(L-glutamic acid)-alanine-paclitaxel conjugate to rats. (C) 2009 Elsevier B.V. All rights reserved.
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