Journal
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Volume 53, Issue 2, Pages 137-144Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jpba.2010.02.001
Keywords
Betula pendula leaves; HPLC-DAD; LC-MS; GC-MS; Validation; Flavonol quantification; Phenolic conversion
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An HPLC method, which allows reliable quantitation of flavonols and other phenolics in birch leaf extracts, was developed and validated. The method was applied to study the bioconversion of flavonols in fermented aqueous extracts. Almost 100% of the flavonols were converted during the 12 months observation period. The generated phenolics as well as consecutive conversion products were identified by HPLC-DAD, LC-MS and GC-MS techniques. (C) 2010 Elsevier B.V. All rights reserved.
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