Journal
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Volume 49, Issue 5, Pages 1166-1171Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jpba.2009.02.024
Keywords
Nicotiana tabacum; GC-FID; Nicotine validated quantification; Amine-deactivated column; GC-MS; Nicotine metabolites
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Funding
- WALA GmbH (Germany)
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A new GC-FID method, which allows rapid and reliable quantitation of nicotine in tobacco leaf extracts, was developed and validated. To avoid nicotine adsorption on the column,an amine-deactivated capillary column was used. The method developed was applied to study the degradation of nicotine in a fermented aqueous extract, and a loss of nearly 20% of nicotine over 12 months was observed. Careful inspection of GC-MS runs from concentrated samples of the same extract revealed the presence of nicotine metabolites such as nornicotine, anatabine, myosmine, 2,T-bipyridyl, and 2-pyrrolidinone. (C) 2009 Elsevier B.V. All rights reserved.
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