SPE-HPTLC of procyanidins from the barks of different species and clones of Salix

A SPE-HPTLC method was developed for the qualitative and quantitative analysis of procyanidin B-1 in willow barks. The chromatography was performed on HPTLC silica gel layer with the mobile phase chloroform-ethanol-formic acid (50:40:6v/v/v), in the Automatic Developing Chamber-ADC 2. The methanol extracts from willow barks were purified by SPE method on RP-18 silica gel columns with methanol-water (7:93 v/v) as the eluent. The presence of procyanidin 131 was revealed in the majority of investigated willow barks. The content of procyanidin 131 varied from 0.26 mg/g in the extract of Salix purpurea clone 1067-2.24 mg/g in the extract of Salix alba clone 1100. The method was validated for linearity, precision, LOD, LOQ and repeatability. (C) 2008 Elsevier B.V. All rights reserved.