Journal
JOURNAL OF PESTICIDE SCIENCE
Volume 38, Issue 1-2, Pages 49-59Publisher
PESTICIDE SCI SOC JAPAN
DOI: 10.1584/jpestics.D12-073
Keywords
gene targeting; herbicide tolerance; point mutation; acetolactate synthase
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Funding
- Ministry of Agriculture, Forestry and Fisheries of Japan (Genomics for Agricultural Innovation) [GMC-0001]
- Bio-Oriented Technology Research Advancement Institution (BRAIN) of Japan
- Budget for Nuclear Research of the Ministry of Education, Culture, Sports, Science and Technology
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Herbicide-tolerant crops including glyphosate-tolerant crops, generated via transgenic approaches are now cultivated widely. However, only a few varieties of transgenic herbicide-tolerant crops have been commercialized due to the time and cost required to obtain regulatory clearance. In contrast, herbicide-tolerant crops resulting from mutagenesis approaches are less problematic. However, the low occurrence of spontaneous mutations at the required sequence in the target locus continues to limit this approach. A promising alternative for inducing desirable mutations in endogenous plant genomes is homologous recombination (HR)-dependent gene targeting (GT). Here, we review three different approaches to create herbicide-tolerant plants, i.e., transgenic approaches, mutagenesis and HR-dependent GT. Among these techniques, HR-dependent GT is relatively new. We introduce the basic mechanism of HR-dependent GT, present an example of how to construct herbicide-tolerant rice plants by this method, and suggest candidate genes that could be targeted to confer herbicide tolerance by HR-dependent GT. (C) Pesticide Science Society of Japan
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