Chemical composition, phytotoxic and antifungal activities of Pinus pinea essential oil

The chemical composition of essential oils isolated by hydrodistillation from the aerial parts of Tunisian Linn. was analyzed by GC and GC/MS. Sixty-six compounds were identified, representing 98.5% of total oil, which was found to be rich in monoterpene hydrocarbons (73.1%) particularly limonene (54.1%), alpha-pinene (7.7%), and beta-pinene (3.4%). The yield and the physico-chemical properties were determined. Results of the antifungal activity study by in vitro contact assay showed that oil significantly inhibited the growth of ten plant pathogenic fungi. Moreover, herbicidal properties of the oil, tested on L., Gaud., and L., indicated that the oil completely inhibited the seed germination at a high concentration, while at low doses the oil acted by decreasing and delaying the germination and inhibiting the seedling growth of all tested weeds unlike the commercial herbicide. Our results showed that essential oil could be valorized as bioproduct for biocontrol of weeds and fungal plant diseases.