Journal
JOURNAL OF PERIODONTOLOGY
Volume 83, Issue 2, Pages 242-247Publisher
AMER ACAD PERIODONTOLOGY
DOI: 10.1902/jop.2011.110177
Keywords
Beta-defensin 2; human; beta-defensin 3; human; epithelium; Fusobacterium nucleatum; LL-37 peptide
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Funding
- Universities of Helsinki (HUS-EVO) [T1020Y0008]
- Turku
- Turku University Foundation [388/211/2009]
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Background: In the present study, the expression and localization of three epithelial peptides (human p-defensin [hBD]-2 and -3, and cathelicidin [LL-37]) are studied in an organotypic dento-epithelial (OD-E) model exposed to Fusobacterium nucleatum (Fn) biofilm. Methods: Biofilm of Fn ATCC 25586 or AHN 9508 were produced by culturing each strain on semipermeable membranes. The OD-E model was constructed by seeding keratinocytes on fibroblast-containing collagen gels and by placing dentin pieces on the top. A 3-day-old biofilm was placed on the top of the OD-E and the coculture was incubated for 5 hours or 24 hours. Production of epithelial antimicrobial peptides was determined immunohistochemically. Results: After 5 hours of incubation, the biofilm of each Fn strain stimulated expression of hBD-2 and -3. hBD-2 was localized on superficial layers and hBD-3 on basal cell layers of the epithelium and dento-epithelial junctions, whereas LL-37 was only weakly expressed. After 24 hours, hBD-2 expression was extended toward basal cell layers of the epithelium. In contrast, hBD-3 expression extended toward superficial layers of the epithelium. In the case of Fn AHN 9508 biofilm, LL-37 was localized in the cell layers of the dento-epithelial junction. Conclusion: In our OD-E model, epithelial antimicrobial peptide responses to Fn biofilms have distinct regulation and localization characteristics, resembling those known to occur in the gingival epithelium in vivo. J Periodontot 2012;83:242-247.
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