Journal
JOURNAL OF PEPTIDE SCIENCE
Volume 20, Issue 8, Pages 630-639Publisher
WILEY
DOI: 10.1002/psc.2640
Keywords
Huntington's disease; polyglutamine aggregation; peptide inhibitor; nanoprecipitation; peptide encapsulation
Funding
- Department of Biotechnology, Government of India
- Indian Institute of Technology (IIT) Kanpur
Ask authors/readers for more resources
Huntington's and eight other neurodegenerative diseases occur because of CAG repeat expansion mutation culminating into an expanded polyglutamine tract in respective protein. In Huntington's disease (HD), a number of CAG repeats beyond normal repeat length (>36) lead to the formation of mutant protein, the proteolytic cleavage of which induces aggregation in polyglutamine length-dependent manner. The neurodegeneration in this disease is linked to aggregation, and its inhibition is a potential approach for therapeutic development. Although peptides and other molecules have been developed for inhibiting aggregation, peptides in general are susceptible to degradation in vivo conditions. To understand their clinical significance, they also need to be delivered through blood-brain barrier. Here, for the first time, we have synthesized poly-D,L-lactide-co-glycolide nanoparticles containing a polyglutamine aggregation inhibitor peptide PGQ(9)[P-2], by nanoprecipitation method. This process yielded less than 200nm spherical nanoparticles with uniform distribution. Characterization studies by infrared spectroscopy-based and HPLC-based assays show the presence of PGQ(9)[P-2] in nanoparticles. In vitro release kinetics demonstrates that nanoparticles release PGQ(9)[P-2] by erosion and diffusion processes. When the PGQ(9)[P-2]-loaded nanoparticles are incubated with aggregation-prone Q(35)P(10) peptide, representing N-terminal part of Huntingtin protein, it arrests the elongation phase of Q(35)P(10) aggregation. These findings propose the first step toward delivery of a peptide inhibitor against polyglutamine aggregation in HD. Copyright (C) 2014 European Peptide Society and John Wiley & Sons, Ltd.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available