Journal
JOURNAL OF PEPTIDE SCIENCE
Volume 17, Issue 6, Pages 445-453Publisher
WILEY-BLACKWELL
DOI: 10.1002/psc.1342
Keywords
quaternary ammonium salts; derivatization of peptides; peptide fragmentation; sequencing
Funding
- Ministry of Science and Higher Education of Poland [N N401 222734]
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A series of model peptides in the form of quaternary ammonium salts at the N-terminus was efficiently prepared by the solid-phase synthesis. Tandem mass spectrometric analysis of the peptide quaternary ammonium derivatives was shown to provide sequence confirmation and enhanced detection. We designed the 2-(1,4-diazabicyclo[2.2.2] octylammonium) acetyl quaternary ammonium group which does not suffer from neutral losses during MS/MS experiments. The presented quaternization of 1,4-diazabicyclo[2.2.2] octane (DABCO) by iodoacetylated peptides is relatively easy and compatible with standard solid-phase peptide synthesis. This methodology offers a novel sensitive approach to analyze peptides and other compounds. Copyright (C) 2011 European Peptide Society and John Wiley & Sons, Ltd.
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