Journal
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
Volume 100, Issue 9, Pages 3909-3921Publisher
SPRINGER
DOI: 10.1007/s00253-015-7235-4
Keywords
Cordyceps militaris; Intracellular polysaccharides; Biosynthesis; Enzyme activity
Categories
Funding
- National Spark Key Program of China [2015GA610001]
- Foundation of Tianjin University of Science and Technology [20120106]
- International Science and Technology Cooperation Program of China [2013DFA31160]
- Foundation of Tianjin Educational Committee [20090604]
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The influence of different fermentation conditions on intracellular polysaccharide (IPS) production and activities of the phosphoglucomutase (PGM), UDPG-pyrophosphorylase (UGP), phosphoglucose isomerase (PGI), UDPG-dehydrogenase (UGD), and glucokinase (GK) implicated in metabolite synthesis in Cordyceps militaris was evaluated. The highest IPS production (327.57 +/- 6.27 mg/100 mL) was obtained when the strain was grown in the optimal medium containing glucose (40 g center dot L-1), beef extract (10 g center dot L-1), and CaCO3 (0.5 g center dot L-1), and the initial pH and temperature were 7 and 25 A degrees C, respectively. The activities of PGM, UGP, and PGI were proved to be influenced by the fermentation conditions. A strong correlation between the activities of these enzymes and the production of IPS was found. The transcription level of the pgm gene (encoding PGM) was 1.049 times and 1.467 times compared to the ugp gene and pgi gene (encoding UGP and PGI), respectively, in the optimal culture medium. This result indicated that PGM might be the highly key enzyme to regulate the biosynthesis of IPS of C. militaris in a liquid-submerged culture. Our study might be helpful for further research on the pathway of polysaccharide biosynthesis aimed to improve the IPS production of C. militaris.
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