Journal
JOURNAL OF PARASITOLOGY
Volume 95, Issue 1, Pages 75-81Publisher
ALLEN PRESS INC
DOI: 10.1645/GE-1634.1
Keywords
-
Categories
Funding
- Canadian Water Network (CWN) Center of Excellence
- Natural Sciences and Engineering Research Council (NSERC) of Canada
- Ontario Ministry of Agriculture and Food (OMAF)
- Ontario Ministry of Environment
- Canadian Foundation for Innovation
- Ontario Innovation Trust
- NSERC and OGS
Ask authors/readers for more resources
Cryptosporidium parvum, an apicomplexan parasite transmitted via animal fecal wastes, is the causative agent of cryptosporidiosis. Clones were selected from 2 synthetic nave human single-chain variable fragment (scFv) phagemid libraries that bound to the recombinant P23 protein of C. parvum. Panning the Tomlinson I and J phagemid libraries resulted in 6 distinct clones. Two clones had full-length scFv sequences, while the remaining clones were either truncated or missing a section of the heavy chain. Despite these differences, all clones were able to detect both native C. parvum proteins and recombinant P23. None of the selected clones cross-reacted with Escherichia coli, Streptococcus pyogenes, Listeria monocytogenes, Bacillus cereus, Giardia lamblia (cysts or trophozoites), or with S16, another dominant surface antigen on C. parvum sporozoites. Clones expressed as the scFv-gIIIp fusion construct in soluble form detected C. parvum. Panning from naive libraries is a useful method for isolation and identification of recombinant antibodies that have the potential for use in pathogen detection and immunotherapy.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available