Journal
JOURNAL OF ORTHOPAEDIC RESEARCH
Volume 27, Issue 11, Pages 1493-1498Publisher
WILEY
DOI: 10.1002/jor.20896
Keywords
platelet rich plasma; vascular endothelial growth factor; bone marrow stromal cells; endothelial cells; bone repair
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Funding
- Fondazione Monte dei Paschi of Siena
- Regione Emilia-Romagna Progetto di Ricerca Regione-Universita: Regenerative Medicine in Osteo-articular Diseases
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Platelet-rich plasma (PRP) is used to accelerate bone repair through the growth factors released by platelets. The purpose of this study was to evaluate if PRP induce human umbilical vein endothelial cells (HUVEC) to express mRNA for osteogenic growth factors and stimulate the migration of bone marrow stromal Cell (BMSC). The effects of PRP were compared to those induced by vascular endothelial growth factor-A (VEGF-A) or, as a negative control, by platelet poor plasma (PPP). After incubation with PRP, but not with PPP, HUVEC showed an increased expression of mRNA for platelet derived growth factor-B (PDGF-B), and this effect was not inhibited by an anti-VEGF-A antibody. The migration of BMSC was more stimulated by HUVEC incubated with PRP than by HUVEC incubated with low serum medium or PPP. Besides, PRP increased the expression of intercellular adhesion molecule-1 (ICAM-1.) and osteoprotegerin, but did not affect the expression either of the receptor activator for nuclear factor kappa B ligand (RANKL) or of RANK. These findings support the hypothesis that PRP contribute to bone repair by favoring the pro-osteogenic function of endothelial cells, including the recruitment of osteoblast precursors and the expression of adhesion molecules for monocyte/macrophages, while inhibiting their pro-osteolytic properties. (C) 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 27:1493-1498, 2009
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