Production of carcinogenic acetaldehyde by Candida albicans from patients with potentially malignant oral mucosal disorders

Objectives Production of carcinogenic acetaldehyde by Candida has been suggested to contribute to epithelial dysplasia and oral carcinogenesis. Oral lichen planus (OLP), oral lichenoid lesion (OLL) and oral leukoplakia (OL) are potentially carcinogenic oral diseases where colonisation by Candida is common, but acetaldehyde production by Candida has not been studied. Study Design Acetaldehyde production in ethanol (11mM), glucose (100mM), ethanolglucose (11mM and 100mM) or red wine (1200mM ethanol) incubation by Candida albicans from patients with OLL (n=6), OLP (n=16), OL (n=6) and controls (n=6) was measured by gas chromatography. Participants completed a questionnaire regarding their smoking habits and alcohol consumption. Results All Candida albicans isolates produced potentially carcinogenic levels of acetaldehyde (>100M) in all incubations containing ethanol. The control group isolates produced the highest acetaldehyde levels. Isolates from smokers produced more acetaldehyde in all incubations than those from non-smokers. The difference was significant in ethanolglucose incubation. Isolates from patients who were both smokers and drinkers produced the highest amounts when incubated in ethanol, ethanolglucose and wine. Conclusions Candida albicans isolated from potentially carcinogenic oral diseases can produce mutagenic amounts of acetaldehyde. Cigarette smoking and alcohol consumption may favour adaptational changes resulting in the upregulation of candidal acetaldehyde metabolism. J Oral Pathol Med (2013) 42: 243-249