Journal
JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY
Volume 54, Issue 4, Pages 298-302Publisher
CENTER ACADEMIC PUBL JAPAN
DOI: 10.3177/jnsv.54.298
Keywords
PPAR alpha; PPAR delta; arachidonic acid; p300; Caco-2
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Funding
- JSPS [04022]
- Ministry of Education, Culture, Sports, Science and Technology of Japan [18590220, 18790171]
- Grants-in-Aid for Scientific Research [18590220, 18790171] Funding Source: KAKEN
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It is reported that arachidonic acid strongly induces the conformational change in vitro and transactivity of PPAR alpha in colorectal cancer cell line Caco-2. In this Study. we demonstrated that the induction of conformational change and transactivity of PPAR delta by arachidonic acid. as well as other polyunsaturated fatty acids, was considerably lower than that of PPAR alpha. Mammalian two-hybrid assay showed that arachidonic acid enhanced binding of one of the coactivators. p300, to PPAR alpha but not to PPAR delta. Additionally, arachidonic acid induced in vitro binding of both PPAR alpha-RXR alpha and PPAR delta-RXR alpha heterodimers to several PPREs on CRBPII, L-FABP and ACO genes. Our results suggest that the lower transactivity of PPAR delta for arachidonic acid in Caco-2 cells. compared with PPARa, is associated with the binding activity of p300 to the receptor.
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