Journal
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
Volume 25, Issue 4, Pages 456-462Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jnutbio.2013.12.006
Keywords
Aspartate; Intestine; Energy status; Weanling piglets; Lipopolysaccharide
Funding
- National Natural Science Foundation of China [31372318, 31172222]
- Program for New Century Excellent Talents in University [NCET-10-0158]
- National Basic Research Program of China [2012CB126305]
- Natural Science Foundation of Hubei Province [2010CDA050, 2013CFA029]
- Project of International Cooperation of Hubei Province [2011BFA008]
- Wuhan Science and Technology Program [201171034320]
Ask authors/readers for more resources
The intestine has a high requirement for ATP to support its integrity, function and health, and thus, energy deficits in the intestinal mucosa may play a critical role in intestinal injury. Aspartate (Asp) is one of the major sources of ATP in mammalian enterocytes via mitochondrial oxidation. We hypothesized that dietary supplementation of Asp could attenuate lipopolysaccharide (LPS)-induced intestinal damage via modulation of intestinal energy status. Twenty-four weanling piglets were allotted to one of four treatments: (1) nonchallenged control, (2) LPS-challenged control, (3) LPS+0.5% Asp treatment, and (4) LPS+1.0% Asp treatment. On day 19, pigs were injected with saline or LPS. At 24 h postinjection, pigs were killed and intestinal samples were obtained. Asp attenuated LPS-induced intestinal damage indicated by greater villus height and villus height/crypt depth ratio as well as higher RNA/DNA and protein/DNA ratios. Asp improved intestinal function indicated by increased intestinal mucosal disaccharidase activities. Asp also improved intestinal energy status indicated by increased ATP, ADP and total adenine nucleotide contents, adenylate energy charge and decreased AMP/ATP ratio. In addition, Asp increased the activities of tricarboxylic acid cycle key enzymes including citrate synthase, isocitrate dehydrogenase and alpha-oxoglutarate dehydrogenase complex. Moreover, Asp down-regulated mRNA expression of intestinal AMP-activated protein kinase alpha 1 (AMPK alpha 1), AMPK alpha 2, silent information regulator 1 (SIRT1) and peroxisome proliferator activated receptor gamma coactivator-1 alpha (PGC1 alpha) and decreased intestinal AMPK alpha phosphorylation. These results indicate that Asp may alleviate LPS-induced intestinal damage and improve intestinal energy status. (C) 2014 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available