Journal
JOURNAL OF NUTRITION
Volume 142, Issue 9, Pages 1621-1625Publisher
AMER SOC NUTRITION-ASN
DOI: 10.3945/jn.112.164814
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Funding
- NIH [R37DDK36823, R37DDK36823-26S1, RO1DK079892, R01DK079892-26S1]
- Arkansas Biosciences Institute
- Arkansas Tobacco Settlement Proceeds Act
- National Center for Research Resources [UL1RR029884]
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This work describes a novel liquid chromatography tandem MS (LC-MS/MS) method for the determination of ratios of acylcarnitines arising from acyl-CoA substrates and products that reflect metabolic disturbances caused by marginal biotin deficiency. The urinary ratios reflecting reduced activities of biotin-dependent enzymes include the following: 1) the ratio of 3-hydroxyisovalerylcarnitine : 3-methylglutarylcarnitine (3HIAc : MGc) for methylcrotonyl-CoA carboxylase; 2) the ratio of propionylcarnitine:methylmalonylcarnitine (Pc : MMc) for propionyl-CoA carboxylase (PCC); and 31 the ratio of acetylcarnitine : malonylcarnitine (Ac: Mc) for acetyl-CoA carboxylase. To demonstrate the suitability of the LC-MS/MS method for biomonitoring, we measured the 3 ratios for 7 healthy adults at various time points (d o, 14, and 28) during the induction of marginal biotin through the consumption of egg white. The mean change in the Pc: MMc ratio relative to d 0 was 5.3-fold by d 14 (P = 0.0049) and 8.5-fold by d 28(P = 0.00421. The mean change in the 3HIAc : MGc ratio was 2.8-fold by d 14 (P = 0.0022) and 3.8-fold by d 28 (P =0.0001). The mean change in the Ac: Mc ratio was 2.9-fold by d 14 (P = 0.03) and 4.7-fold by d 28 (P = 0.02). The results suggest that simultaneous assessment of ratios of multiple biotin-dependent pathways offers insight into the complex metabolic disturbances caused by marginal biotin deficiency. We hypothesize that one or a combination of the ratios might be more sensitive or robust with respect to other nutrient deficiencies or confounding metabolic processes. J. Nutr. 142: 1621-1625, 2012.
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