Journal
JOURNAL OF NON-CRYSTALLINE SOLIDS
Volume 356, Issue 31-32, Pages 1576-1580Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jnoncrysol.2010.05.054
Keywords
Biopolymer B125; Differential scanning calorimetry D190; Mass spectrometry M105; Thermal analysis T135
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Among technologies for melanin commercial-scale production, microbial synthesis is an attractive option. In this study, a melanin producer, wild strain Bacillus subtilis, was isolated from the soil, and pigment from this bacterium was purified and characterised using different techniques. The use of tandem pyrolysis/gas chromatography/mass spectrometry allowed us to classify the pigment as a sulphur-containing bacterial melanin. Thermogravimetric and differential thermal analyses indicated that melanin might contain two fractions of water, a strongly bound fraction and a weakly-bound fraction. Scanning electron microscopy showed an amorphous structure without differential characteristics, and electronic conductivity measurements at normal atmospheric conditions and humidities suggest that melanin may be an insulator. However, more work is underway to clarify this point. (C) 2010 Elsevier B.V. All rights reserved.
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