Journal
JOURNAL OF NEUROSCIENCE RESEARCH
Volume 90, Issue 10, Pages 2034-2042Publisher
WILEY-BLACKWELL
DOI: 10.1002/jnr.23081
Keywords
frontotemporal lobar degeneration; p62; SQSTM1; TAR DNA-binding protein of 43 kDa (TDP-43); ubiquitin
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Funding
- Ministry of Education, Culture, Sports, Science, and Technology, Japan [23500425, 23500424, 20300123]
- National Natural Science Foundation of China [81100961]
- Hirosaki University Institutional Research
- Karoji Memorial Fund for Medical Research
- Brain Research Institute, University of Niigata [2011-2209]
- Intramural Research Grant for Neurological and Psychiatric Disorders of NCNP [21B-4]
- Research Committee for CNS Degenerative Diseases, Ministry of Health, Labor and Welfare, Japan
- Grants-in-Aid for Scientific Research [23500425, 23659184, 23500424, 20300123] Funding Source: KAKEN
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Ubiquitin-positive cytoplasmic inclusions are consistently found in various neurodegenerative diseases. As with ubiquitin, anti-p62/SQSTM1 (referred to as p62) antibody clearly immunostains these inclusions. p62 has a ubiquitin-associated domain at the carboxyl terminus and thereby interacts with ubiquitinated and misfolded proteins. Here we immunoprecipitated endogenous p62 in the cerebral cortex from patients with frontotemporal lobar degeneration with TDP-43 inclusions (FTLD-TDP) and found that p62 coimmunoprecipitated several proteins, including TDP-43, which is a major disease protein in FTLD-TDP. Unexpectedly, p62 immunoprecipitated a smaller amount of TDP-43 in FTLD-TDP compared with controls. Further analyses showed that p62 physiologically binds to TDP-43 and likely is involved in degradation of TDP-43 with 35-kDa, but not full-length TDP-43. Our results suggest that the interaction of TDP-43 and p62 is disrupted and may participate in the pathogenesis of TDP-43 proteinopathy. (c) 2012 Wiley Periodicals, Inc.
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