Journal
JOURNAL OF NEUROSCIENCE RESEARCH
Volume 89, Issue 2, Pages 199-211Publisher
WILEY-BLACKWELL
DOI: 10.1002/jnr.22538
Keywords
glioma; microglia; chemokine; ATP; purinergic receptor
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Funding
- National Science Counci of Taiwan [NSC 98-2321-B-006-002]
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Chemokines can be produced by gliomas, which mediate the infiltration of microglia, a characteristic feature of glioma-associated neuropathogenesis. ATP that is released at a high level from glioma has been reported to play a regulatory role in chemokine production in cultured glioma cells. The objective of this study was to define the potential role of extracellular ATP in the regulation of macrophage inflammatory protein-1 alpha (MIP-1 alpha) and monocyte chemoattractant protein-1(MCP-1) expression in glioma-associated microglia/macrophages. The results showed that Iba1(+) and ED1(+) microglia existed in the tumor at 3 and 7 day after injection of C6 glioma cells into the rat cerebral cortex (dpi). ED1(+) microglia/macrophages or Iba1(+) microglia in the glioma were also colocalized to MIP-1 alpha- and MCP-1-expressing cells. In vitro study indicated that treatment with ATP and BzATP (an agonist for ATP ionotropic receptor P2X(7)R) caused an increase in the intracellular levels of microglial MIP-1 alpha and MCP-1. By using an extracellular Ca2+ chelator (EGTA) and P2X(7)R antagonists, oxidized ATP (oxATP) and brilliant blue G (BBG), we demonstrated that BzATP-induced production of MIP-1 alpha and MCP-1 levels was due to P2X(7)R activation and Ca2+-dependent regulation. Coadministration of C6 glioma cells and oxATP into the rat cerebral cortex resulted in a reduction of MIP-1 alpha- and MCP-1-expressing microglia/macrophages. We suggest, based on the results from in vivo and in vitro studies, that a massive amount of ATP molecules released in the glioma tumor site may act as the regulator with P2X(7)R signaling that increases MIP-1 alpha and MCP-1 expression in tumor-infiltrating microglia/macrophages. (C) 2010 Wiley-Liss, Inc.
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