Journal
JOURNAL OF NEUROSCIENCE METHODS
Volume 209, Issue 2, Pages 367-370Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jneumeth.2012.06.019
Keywords
Rabies; Transneuronal tracing; Transsynaptic tracing; Tract-tracing; Retrograde labeling
Categories
Funding
- US National Institute of Health [DC-04199, RR-018604, NS-043277]
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The transynaptic and retrograde spread of rabies virus make it an efficient and robust transneuronal tracer, capable of revealing connectivity patterns of multisynaptic, neuronal circuits with great detail. Current techniques begin by infecting many neurons simultaneously, from which higher-order neurons are then labeled sequentially in time. Here we report on a method that can initially infect a single neuron-of-choice, allowing for greater precision and specificity of labeled circuits. (C) 2012 Elsevier B.V. All rights reserved.
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