Journal
JOURNAL OF NEUROSCIENCE METHODS
Volume 177, Issue 1, Pages 122-130Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jneumeth.2008.10.006
Keywords
Immunofluorescence; Cell proliferation; Neurogenesis; Neural precursor; Cell differentiation; Thymidine analogue; Cell labelling; Brain
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Funding
- Australian Department of Health and Ageing
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Labelling and identifying proliferating cells is central to understanding neurogenesis and neural lineages in vivo and in vitro. We present here a novel thymidine analogue, ethynyl deoxyuridine (EdU) for labelling dividing cells, detected with a fluorescent azide which forms a covalent bond via the click chemistry reaction (the Huisgen 1,3-dipolar cycloaddition reaction of an organic azide to a terminal acetylene). Unlike the commonly used BrdU, EdU detection requires no heat or acid treatment. It is quick and easy and compatible with multiple probes for fluorescence immunochemistry, facilitating the characterisation of proliferating cells at high resolution. (C) 2008 Elsevier B.V. All rights reserved.
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