Journal
JOURNAL OF NEUROSCIENCE METHODS
Volume 182, Issue 2, Pages 157-164Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jneumeth.2009.06.002
Keywords
Immunohistochemistry; GFP expression; HeLa cells; Formaldehyde fixation; Antibodies; Double labeling; Hippocampus; Cell culture
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Funding
- NIH NS [44062]
- NSF [0447192]
- CSU Chico Research Foundation
- Direct For Biological Sciences
- Division Of Integrative Organismal Systems [0447192] Funding Source: National Science Foundation
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A better understanding of improved microwave technology has increased the benefits and versatility of the technique as it applies to all aspects of immunohistochemistry. The role of continuous magnetron power output (wattage) combined with precise control of sample heating demonstrated their significance to complex labeling protocols. Here, we present results for microwave-assisted formaldehyde fixation and its effect on GFP expression in transfected HeLa cells. Rat brain sections and cultured hippocampal cells were labeled with 11 different primary antibodies using a unified microwave protocol. Microwave-assisted immunohistochemistry made it possible to sequentially label tissues and cells with several primary antibodies in a very short period of time with excellent labeling characteristics. (C) 2009 Elsevier B.V. All rights reserved.
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