Journal
JOURNAL OF NEUROSCIENCE METHODS
Volume 184, Issue 1, Pages 25-36Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jneumeth.2009.07.015
Keywords
Immediate early genes; Arc; Arg3.1; Green fluorescent protein (GFP); Transgenic mice; Synaptic plasticity; Neural circuits; Stress
Categories
Funding
- Max-Planck Society, Human FrontierScience Program [RGY0079/2005-C]
- Alzheimer Association [NIRG-05-14907]
Ask authors/readers for more resources
The brain-specific immediate early gene Arc/Arg3.1 is induced in response to a variety of stimuli, including sensory and behavior-linked neural activity. Here we report the generation of transgenic mice, termed TgArc/Arg3.1-d4EGFP, expressing a 4-h half-life form of enhanced green fluorescent protein (d4EGFP) under the control of the Arc/Arg3.1 promoter. We show that d4EGFP-mediated fluorescence faithfully reports Arc/Arg3.1 induction in response to physiological, pathological and pharmacological stimuli, and that this fluorescence permits electrical recording from activated neurons in the live mouse. Moreover, the fluorescent Arc/Arg3.1 indicator revealed activity changes in circumscribed brain areas in distinct modes of stress and in a mouse model of Alzheimer's disease. These findings identify the TgArc/Arg3.1-d4EGFP mouse as a versatile tool to monitor Arc/Arg3.1 induction in neural circuits, both in vitro and in vivo. (C) 2009 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available