Journal
JOURNAL OF NEUROSCIENCE METHODS
Volume 169, Issue 1, Pages 1-7Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jneumeth.2007.11.011
Keywords
spinal cord; microglia; axons; in vivo imaging; two-photon microscopy
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Funding
- NCRR NIH HHS [P41 RR004050, RR04050] Funding Source: Medline
- NINDS NIH HHS [R01 NS052189, R01 NS051470, NS051470, F32 NS056697, NS052189, P30 NS047101, F32 NS056697-02, R01 NS054734] Funding Source: Medline
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In vivo imaging has revolutionized our understanding of biological processes in brain physiology and pathology. However, breathing-induced movement artifacts have impeded the application of this powerful too] in studies of the living spinal cord. Here we describe in detail a method to image stably and repetitively, using two-photon microscopy, the living spinal tissue in mice with dense fluorescent cells or axons, without the need for animal intubation or image post-processing. This simplified technique can greatly expand the application of in vivo imaging to study spinal cord injury, regeneration, physiology and disease. (c) 2007 Elsevier B.V. All rights reserved.
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